Triskelion ( clathrin)
The triskelion shape of the clathrin molecule enables it to form the polyhedral protein network that covers clathrin-coated pits and vesicles. Domains within the clathrin heavy chain that are responsible for maintaining triskelion shape and function were identified and localized. Sequences that mediate trimerization are distal to the carboxyl terminus and are adjacent to a domain that mediates both light chain binding and clathrin assembly. Structural modeling predicts that within this domain, the region of heavy chain-light chain interaction is a bundle of three or four alpha helices.
Sandwich ELISA Isotype detection
Sandwich ELISA is performed to measure the amount and serological class of antibodies made by an immunized animal or present in the serum of patients. Anti-immunoglobulin antibodies is used as the specific and sensitive agents of detection.
