Mulitplex Microarray Hybridization

ArrayIt® has developed an innovative solution to multi-well microarrays that includes glass substrate slides containing a hydrophobic mask for well creation and a submersible hybridization cassette that allows temperature-controlled reactions without sample evaporation. Twenty four microarrays in a 3 x 8 pattern can be printed, reacted, scanned and quantified on a single glass substrate slide, offering highly affordable sample multiplexing for research and diagnostics.



Designed for up to 24 high or low biochemical reactions on a single microarray substrate or slide. The unit is air tight to prevent evaporation and water proof, therefore short and extended reactions can be run in a high or low temperature controlled water bath or oven. This 24 well submersible hybridization cassette fits one 24 well masked substrate for 24 separate microarray reactions using a single cassette. No cover slips required.

This submersible cassette is made from a durable, flat, precision-machined corrosion resistant alloy and high quality black rubber gasket to ensure proper alignment and no leaking from “well to well”.

For more Information Visit:http://www.arrayit.com/Products/Microarray_Tools/Multiplex_Microarrays/multiplex_microarrays.html

Microarray Hybridization

Microarrays allows the identification of organisms in an ecological sample by hybridizing DNA extracted from the sample to a set of known DNA specimens arrayed on a solid support, usually a microscope slide (reviewed in Call et al. 2003). The sample DNA, or more usually PCR product derived from it, is labeled with a fluorophore so that the specific sites of hybridization on the microarray can be visualized and the intensity of the hybridization signal quantified. The method is conceptually identical to Southern hybridization of a dot blot, but technological innovations allow thousands of known DNA samples to be arrayed on a single microscope slide and many such slides to be produced in a single batch at low cost.