Genechip Animation

Definition

An array of short DNA oligonucleotides (usually 20 nucleotides long) synthesized onto a solid support by photolithography, each of which corresponds to a single gene.

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Stephen Fodor developed a technique called GeneChip probe arrays where he built the sequences he wanted. These GeneChip arrays are printed on special glass.DNA Sequence are built up using light directed chemical synthesis. First, a substrate with a nucleotide is fixed onto the chip at specific positions.The nucleotide has a protecting group(X) that blocks polymerization. This protector group is photolabile and is released on exposure to UV light. Without the protector, polymerization and chain build-up occur.A filter is added to the chip so that only some of the nucleotides are exposed to light. These deprotected groups are then free to add the next nucleotide to the chain.By alternating the position of the filter, Fodor can build a GeneChip with an array of different sequences about 20 nucleotides long.Fodor added a cDNA probe to the GeneChip.He could simultaneously assay tens of thousands of different sequences at the same time.

Mulitplex Microarray Hybridization

ArrayIt® has developed an innovative solution to multi-well microarrays that includes glass substrate slides containing a hydrophobic mask for well creation and a submersible hybridization cassette that allows temperature-controlled reactions without sample evaporation. Twenty four microarrays in a 3 x 8 pattern can be printed, reacted, scanned and quantified on a single glass substrate slide, offering highly affordable sample multiplexing for research and diagnostics.



Designed for up to 24 high or low biochemical reactions on a single microarray substrate or slide. The unit is air tight to prevent evaporation and water proof, therefore short and extended reactions can be run in a high or low temperature controlled water bath or oven. This 24 well submersible hybridization cassette fits one 24 well masked substrate for 24 separate microarray reactions using a single cassette. No cover slips required.

This submersible cassette is made from a durable, flat, precision-machined corrosion resistant alloy and high quality black rubber gasket to ensure proper alignment and no leaking from “well to well”.

For more Information Visit:http://www.arrayit.com/Products/Microarray_Tools/Multiplex_Microarrays/multiplex_microarrays.html

Microarray centrifugation

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